PHYTOCHEMICAL INVESTIGATION OF HAPLOPHYLLUM PEDICELLATUM BUNGE ET BOISS AND IDENTIFICATION OF QUINOLINE ALKALOIDS

Jorabek Qulmatov; Salixjan Maulyanov; Mubashshirxon Eshonov

Authors

Jorabek Qulmatov Mirzo Ulug‘bek nomidagi O‘zbekiston Milliy Universiteti Kimyo fakulteti, Tabiiy birikmalar va amaliy kimyo kafedrasi o‘qituvchisi,
Salixjan Maulyanov Mirzo Ulug‘bek nomidagi O‘zbekiston Milliy Universiteti Kimyo fakulteti, Tabiiy birikmalar va amaliy kimyo kafedrasi dotsenti
Mubashshirxon Eshonov Mirzo Ulug‘bek nomidagi O‘zbekiston Milliy Universiteti Kimyo fakulteti, Tabiiy birikmalar va amaliy kimyo kafedrasi dotsen vazifasini bajaruvchisi, PhD,

Keywords:

Haplophyllum pedicellatum, Rutaceae, quinoline alkaloids, furoquinoline alkaloids, Pedicin, phytochemistry, medicinal plants, N-methyl-2-phenylquinolin-4-one, flindersine.

Abstract

Haplophyllum pedicellatum Bunge et Boiss is a perennial medicinal plant belonging to the Rutaceae family and widely distributed in Central Asia. The present study was conducted to investigate the phytochemical composition of H. pedicellatum collected from the Bobotog region of Surkhandarya Province, Uzbekistan. The aerial parts were extracted with 80% ethanol under ultrasonic conditions and subsequently fractionated using solvents of increasing polarity. Chromatographic separation of the ethyl acetate fraction yielded several quinoline alkaloids, including furoquinoline derivatives and pyraquinolin-2-one compounds. A previously undescribed alkaloid, designated Pedicin, was isolated and characterized. The roots were extracted with methanol, yielding a total alkaloid content of 0.65%. Further chromatographic purification resulted in the isolation of N-methyl-2-phenylquinolin-4-one and flindersine. Structural elucidation was carried out using UV, IR, ESI-MS, TLC, HPLC, melting point analysis, and NMR spectroscopy. The results demonstrate that H. pedicellatum is a valuable natural source of biologically active quinoline alkaloids with potential pharmaceutical applications.

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