Multidisciplinary Journal of Science and Technology

“MOLECULAR GENETIC STUDIES OF THE BUSHUEV BREED OF CATTLE IN UZBEKISTAN”

Giyasov Khusanjon Abdullayevich

Head of the Cattle Breeding Department of the Research Institute Livestock and Poultry Science, Ph.D. agricultural Sciences, senior researcher

##semicolon## Bushuev breed, gene pool, molecular genetic research, DNA, markers, genetic passport.

सार

Preliminary results of molecular genetic studies of the Bushuev cattle breed, which is on the verge of extinction, are presented as part of the scientific and practical project “Development of molecular panels for genetic certification of unique breeds of animals.”The project is being carried out with the aim of preserving the valuable gene pool of the Bushuev breed, the only one bred in Uzbekistan, developing molecular panels for genetic certification and early prediction of breeding and productive qualities of animals.

In molecular genetic studies, which are being carried out on cattle for the first time in the republic, microsatellite DNA markers (short tandem repeats (STR), SNP and InDel markers) will be used.

At the first stage, stud bulls of the state breeding enterprise “Uznaslchilik” were selected for research and used for semen selection. Blood samples were taken from animals. Amplification of DNA fragments was carried out using the Rotor-Gene Q software apparatus from QIAGEN Hilden, Germany. Using invitrogen reagents from Thermo Fisher Scientific (USA), DNA from the blood of Bushuev breed bulls was isolated.Preliminary findings have shown that the higher the level of purebred, the higher the genetic potential of the animals. Final conclusions based on the results of molecular genetic studies will be made after genotyping of animals is completed. Molecular genetic research continues.

##submission.citations##

Amirov Sh. Gapparov Sh., Kuzibaev G. “Bushuev cattle - national wealth”, “Animal husbandry and breeding” № 2, 2020, p. 6-7

Getmantseva O.L., Klimenko A.I. and others. “Molecular genetic research of farm animals using the PCR-RFLP method” Textbook. Don State Agrarian University, Persianovsky, 2018. p.119

Giyasov Kh.A., Boybulov B.Sh. “Let's preserve the only breed of cattle in Uzbekistan”, “Animal husbandry and breeding” Tashkent, 2021. № 03, p. 8-11.

Giyasov Kh.A., “An electronic genetic database of cattle will be developed.” Journal “Agriculture and water management of Uzbekistan”, 2021. № 9, p. 20.

Giyasov Kh.A. and others. “Genetic certification of animals to increase the efficiency of breeding work” Article in the magazine “АGRO NAUKA” 2022. № 2, p. 48-49.

Giyasov Kh.A. Scientific and practical project “Development of molecular panels for genetic certification of unique animal breeds” Tashkent, 2022.

Dankvert S.A., Okhapkin S.K., Dunin I.M. and others. “System of conservation, restoration and rational use of the gene pool of domestic small breeds of cattle” Moscow 2001.

Koltsov D.N. Dmitrieva V.I. and others. “EAF genotypes – blood group systems in cattle breeding for productivity”, “Achievements of science and technology of the agro-industrial complex.” 2019, T.33№10 p.58-61.

Leonova M.A., Kolosov A.Yu., Radyuk A.V. and others. “Promising marker genes for the productivity of farm animals” Moscow 2013.

Marzanova S.N., Devrishov D.A. and Alekseev Ya.I. “A set of primer sequences and allele specific probes for simultaneous gene diagnosis of four mutant kappa casein alleles in cattle.” № RU 2646140. IPC (C12Q1/68).