MODERN METHODS OF EXTRACTING NUCLEIC ACIDS FROM BIOLOGICAL OBJECTS
Barotov Ulugbek Dilshod ugli
Lecturer of the Navoi State Pedagogical Institute
Shomurodov Normurod Parda ugli
Lecturer of the Navoi State Pedagogical Institute
Yaxshiboyeva Damira Tolib qizi
Lecturer of the Navoi State Pedagogical Institute
Sharapova Gulnoza Valentinovna
Lecturer of the Navoi State Pedagogical Institute
Keywords: Nucleic acids, extracting, isolation, biopolymer, white blood cell, cell lysis, elution, cleaning, SDS, centrifugation, chromatography, salting method
Abstract
The study of nucleic acids began many years ago, and each scientist used unique methods in the study. To study nucleic acids, it is necessary to isolate them first. For this reason, several DNA or RNA isolation methods have been created, each of which has its own advantages. This article teaches the most convenient, easiest, most effective methods of extracting nucleic acids.
References
Ralf Dahm. (2005). Friedrich Miescher and the discovery of DNA. Developmental Biology. 278, 274-288;
Valixanov M.N., Dolimova S.N., Umarova G., Mirhamidova P. “Biologik kimyo va molekulyar biologiya (2-qism)” T-2015. 85-90;
Turaqulov Y.X. “Bioximiya” textbook Tashkent-1996. 455-459;
Preetha J Shetty. (2020). The Evolution of DNA Extraction Methods. AJBSR. 8, 39-45;
Chauhan T. (2019). Plant DNA extraction using CTAB buffer. Genetic Education;
Shamsidinova, G. D., Sayfullaev, A. F., Achilova, N. R., & Baratov, U. D. (2021). Prospects for the development of" Education for sustainable ecological development" on the basis of the declaration of Uzbekistan. Asian Journal of Multidimensional Research (AJMR), 10(3), 144-149.